銀耳多糖(TFPS)在抗光老化中的應用研究(下)
銀耳多糖(TFPS)對UVB照射后人永生化角質(zhì)形成細胞(Ha Ca T細胞)的抗光老化作用,旨在為銀耳多糖(TFPS)在具有抗光老化活性的功能護膚品或修復皮膚光損傷治療藥物方面的應用提供經(jīng)驗和數(shù)據(jù)。
研究顯示銀耳多糖(TFPS)預處理可以降低細胞內(nèi)ROS含量,過氧化氫酶、超氧化物歧化酶、谷胱甘肽過氧化物酶等抗氧化物質(zhì)活性顯著增加,還可以通過上調(diào)Nrf2和下調(diào) Keap1表達保護人真皮成纖維細胞[10]。TFPS可通過抑制蛋白激酶B、p38絲裂原活化蛋白激酶、核轉(zhuǎn)錄因子-κB(NF-κB)的激活來降低脂多糖處理的RAW264.7細胞內(nèi)ROS水平,還可以顯著抑制促炎細胞因子的表達[11, 12]。
研究結(jié)果顯示,1~5 mg/m L的銀耳多糖(TFPS)安全性好,可提高Ha Ca T細胞被UVB照射后 降 低 的 細 胞 活 力 , 研 究 結(jié) 果 表 明 銀 耳 多 糖(TFPS)能夠促進起到抗光損傷的作用,推測可能與抗氧化作用相關,具體仍有待后續(xù)研究,而研究結(jié)果為TFPS在具有抗光老化活性的功能護膚品或修復皮膚光損傷治療藥物方面的應用提供了實驗依據(jù)。
參考文獻:
[1] DIRK B,NICOLAE M,ROSWITHA N.Basement membranes in skin: unique matrix structures with diverse functions?[J]. Histochemistry and cell biology,2009,132(01):1-10.
[2] RENATO V I.Basement membrane proteoglycans:from cellar to ceiling[J].Nature reviews Molecular cell biology, 2005, 6(08):646-656.
[3] TERRA V A,SOUZA-NETO F P,FRADE M A,et al.Genistein prevents ultraviolet B radiation-induced nitrosative skin injury and promotes cell proliferation[J].Journal of Photochemistry & Photobiology,B: Biology, 2015, 144:20-27.
[4] DEEVYA L N,RAO N S,JOSHUA L F.Ultraviolet radiation and skin cancer[J].International journal of dermatology, 2010,49(09):978-986.
[5] ASHLEY S,HE Y Y.Mechanisms and prevention of UV-induced melanoma[J].Photodermatology,photoimmunolog & photomedicine,2018,34(01):13-24.
[6] 劉紹東,王秉賢.紫外線照射致小鼠皮基底細胞DNA損傷的劑量-效應研究[J].中國公共衛(wèi)生學報,1993(01):39-40.
[7] HOSSEINI M,DOUSSET L,MAHFOUF W,et al.Energy Metabolism Rewiring Precedes UVB-Induced Primary SkinTumor Formation[J].Cell Reports,2018,23(12):3621-3634.
[8] 安星亮,趙永亮,王歡,等.銀耳多糖提取工藝的響應面法優(yōu)化及抗氧化和保濕性研究[J].食品研究與開發(fā),2022,43(15):123-130.
[9] 李國光.銀耳多糖益生元效應的研究[J].現(xiàn)代食品,2021(03):215-217.
[10] FU H,YOU S,ZHAO D,et al.Tremella fuciformis polysaccharides inhibit UVA-induced photodamage of human dermalfibroblast cells by activating up-regulating Nrf2/Keap1 pathways[J].Journalof Cosmetic Dermatology,2021,20(12):4052-
4059.
[11] RUAN Y,LI H,PU L,et al.Tremella fuciformis Polysaccharides Attenuate Oxidative Stress and Inflammation in MacrophagethroughmiR-155[J].Analyticalcellular pathology(Amsterdam),2018,2018: 5762371.
[12] XIAO H Y, LI H, WEN Y,et al.Tremella fuciformis polysaccharides ameliorated ulcerative colitis via inhibiting
inflammation and enhancing intestinal epithelial barrier function[J].International Journal of Biological Macromolecules,2021,180:633-642.